Plasma Protein Binding Method Comparison Poster at ISSX
Posted on Tue, Nov 01, 2011 @ 07:03 AM
ISSX 17th North American Meeting
The 17th North American Meeting of the ISSX society was held in Atlanta, USA (16-20 October 2011). Presentations were focused on toxicity, transport and enzyme regulation.
Sessions of particular interest included the use of interfering RNA (siRNA) technology and interpretation of the ITC white paper recommendations on the assessment of transporter-mediated interactions.
The presentation of over 300 posters covering diverse topics such as the enzymology (metabolism) of novel compounds, transporter interactions and the latest advances in analytical instrumentation (in particular semi-quantification of metabolites using TOF MS instruments) made for a very interesting and useful meeting.
Plasma Protein Binding Method Comparison
Guy Webber, Chief Scientist at Quotient, presented a poster entitled
"Plasma Protein Binding: A Comparison of Techniques to Help Overcome Common
Issues Encountered During In Vitro Testing"
Plasma protein binding is an integral parameter to assess to aid in the evaluation of pharmacological, pharmacokinetic and toxicological data.
Recent product development in this area has been extensive and there are now different apparatus available to assess plasma protein binding in a well-equipped DMPK laboratory.
This poster shows results using the very latest apparatus, compared to more traditional methods.
Plasma Protein Binding Instrumentation
We used the Fast-Micro-equilibrium DIALYZER™ devices, DispoEquilibrium DIALYZER™ devices, RED™ devices and the HTDialysis 96™ well plate, and compared the binding values obtained with more traditionally encountered techniques such as equilibrium dialysis using Dianorm™ dialysis cells, ultrafiltration (Centrifree™ devices) and ultracentrifugation.
Test compounds with low medium and high plasma protein binding characteristics were used to determine technique limitations and variability.
This work is part of an ongoing validation plan within Quotient Bioresearch designed to help overcome some of the commonly encountered issues that are associated with assessing plasma protein binding in vitro, namely, low throughput, problematic compounds (typically highly lipophilic compounds) and low sample volumes (typically from neonatal plasma).
Which Technique is best for your sample?
Download the poster now to discover more